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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis of HeLa using 68299-1-Ig (same clone as 68299-1-PBS)

    WB analysis of HeLa using 68299-1-Ig (same clone as 68299-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

  • WB analysis of rat testis using 68299-1-Ig (same clone as 68299-1-PBS)

    WB analysis of rat testis using 68299-1-Ig (same clone as 68299-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 68299-1-Ig (same clone as 68299-1-PBS)

    IHC staining of mouse ovary using 68299-1-Ig (same clone as 68299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 68299-1-Ig (LEPREL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

LEPREL2 Monoclonal antibody, PBS Only

LEPREL2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68299-1-PBS

产品说明书

CloneNo. 1F6F3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, Mouse

应用

WB, IHC, Indirect ELISA

别名

GRCB, HSU47926, leprecan like 2, Leprecan like protein 2, LEPREL2, P3H3, Prolyl 3 hydroxylase 3, Protein B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68299-1-PBS targets LEPREL2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Rat, Mouse
免疫原 LEPREL2 fusion protein Ag8850 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 leprecan-like 2
别名 GRCB, HSU47926, leprecan like 2, Leprecan like protein 2, LEPREL2, P3H3, Prolyl 3 hydroxylase 3, Protein B
计算分子量 736 aa, 82 kDa
观测分子量82 kDa
GenBank蛋白编号BC017217
基因名称 LEPREL2
Gene ID (NCBI) 10536
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8IVL6
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.