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验证数据展示

  • IF Staining of human breast cancer using CL594-60066

    IF Staining of human breast cancer using CL594-60066

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 LGALS3BP antibody (CL594-60066, Clone: 2D8E11 ) at dilution of 1:100.

  • IF Staining of human breast cancer using CL594-60066

    IF Staining of human breast cancer using CL594-60066

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 LGALS3BP antibody (CL594-60066, Clone: 2D8E11 ) at dilution of 1:100.

CoraLite®594-conjugated LGALS3BP Monoclonal antibody

LGALS3BP Monoclonal Antibody for IF-P
Cat No. CL594-60066

Print datasheet

CloneNo. 2D8E11

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Synonyms

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 60066-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60066 targets LGALS3BP in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen LGALS3BP fusion protein Ag0294 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name lectin, galactoside-binding, soluble, 3 binding protein
Synonyms 90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K
Calculated Molecular Weight 585 aa, 65 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC002403
Gene Symbol LGALS3BP
Gene ID (NCBI) 3959
RRIDAB_2919892
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08380
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ). LGALS3BP has a predicted mass of ∼65 kDa. It is heavily glycosylated and shows an apparent molecular weight of 90 kDa on SDS-PAGE.

实验方案

Product Specific Protocols
IF protocol for CL594 LGALS3BP antibody CL594-60066Download protocol
Standard Protocols
Click here to view our Standard Protocols