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验证数据展示

  • WB analysis of HepG2 using 21919-1-AP

    WB analysis of HepG2 using 21919-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 21919-1-AP

    IHC staining of human lung using 21919-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 21919-1-AP

    IHC staining of human lung using 21919-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21919-1-AP

    IF Staining of HepG2 using 21919-1-AP

    Immunofluorescent analysis of HepG2 cells using 21919-1-AP (LGI3 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-Rabbit IgG (red).

LGI3 Polyclonal antibody

LGI3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21919-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

UNQ8190/PRO23199, LGIL4, LGI1-like protein 4, LGI1 like protein 4, Leucine-rich repeat LGI family member 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse lung tissue, A549 cells, mouse liver tissue, SMMC-7721 cells
Positive IHC detected inhuman lung tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

21919-1-AP targets LGI3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 LGI3 fusion protein Ag16257 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine-rich repeat LGI family, member 3
别名 UNQ8190/PRO23199, LGIL4, LGI1-like protein 4, LGI1 like protein 4, Leucine-rich repeat LGI family member 3
计算分子量 548 aa, 62 kDa
观测分子量 55-62 kDa
GenBank蛋白编号BC142997
基因名称 LGI3
Gene ID (NCBI) 203190
RRIDAB_10838807
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N145
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LGI3 antibody 21919-1-APDownload protocol
IHC protocol for LGI3 antibody 21919-1-APDownload protocol
IF protocol for LGI3 antibody 21919-1-APDownload protocol
FC protocol for LGI3 antibody 21919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Am J Hum Genet

A reverse genetics and genomics approach to gene paralog function and disease: Myokymia and the juxtaparanode

Authors - Dana Marafi
View Article