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×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 15095-1-AP (same clone as 15095-1-PBS)

    WB analysis using 15095-1-AP (same clone as 15095-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15095-1-AP (LGMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15095-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 15095-1-AP (same clone as 15095-1-PBS)

    IHC staining of mouse kidney using 15095-1-AP (same clone as 15095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15095-1-AP (LGMN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15095-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGMN antibody included
  • IHC staining of mouse kidney using 15095-1-AP (same clone as 15095-1-PBS)

    IHC staining of mouse kidney using 15095-1-AP (same clone as 15095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15095-1-AP (LGMN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15095-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGMN antibody included

LGMN Polyclonal antibody, PBS Only

LGMN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15095-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy LGMN Ready-To-Use IHC Kit. LGMN primary antibody included.

产品信息

15095-1-PBS targets LGMN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 LGMN fusion protein Ag7170 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 legumain
别名
计算分子量 49 kDa
观测分子量 36 kDa
GenBank蛋白编号BC003061
基因名称 LGMN
Gene ID (NCBI) 5641
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99538
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).