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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated LHCGR Polyclonal antibody

LHCGR Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-26424

Print datasheet

Synonyms

FLJ41504, LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing hormone receptor

验证数据展示

  • IF Staining of mouse ovary using CL647-26424

    IF Staining of mouse ovary using CL647-26424

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using CoraLite® Plus 647 LHCGR antibody (CL647-26424) at dilution of 1:200.

  • IF Staining of mouse ovary using CL647-26424

    IF Staining of mouse ovary using CL647-26424

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using CoraLite® Plus 647 LHCGR antibody (CL647-26424) at dilution of 1:200.

经过测试的应用

Positive IF detected inmouse ovary tissue
For other applications, we recommend the unconjugated version of this antibody, 26424-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-26424 targets LHCGR in IF applications and shows reactivity with human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse
Immunogen LHCGR fusion protein Ag19259 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LHCGR
Synonyms FLJ41504, LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing hormone receptor
Calculated Molecular Weight 699 aa, 79 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC157028
Gene Symbol LHCGR
Gene ID (NCBI) 3973
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22888
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LHCGR, also known as LHR or LSHR, belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 LHCGR antibody CL647-26424Download protocol
FC protocol for CL Plus 647 LHCGR antibody CL647-26424Download protocol
Standard Protocols
Click here to view our Standard Protocols