验证数据展示
经过测试的应用
Positive IF-P detected in | mouse ovary tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-26424 targets LHCGR in IF-P applications and shows reactivity with human, mouse samples.
经测试应用 | IF-P Application Description |
经测试反应性 | human, mouse |
免疫原 | LHCGR fusion protein Ag19259 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | LHCGR |
别名 | FLJ41504, LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing hormone receptor |
计算分子量 | 699 aa, 79 kDa |
观测分子量 | 100 kDa |
GenBank蛋白编号 | BC157028 |
基因名称 | LHCGR |
Gene ID (NCBI) | 3973 |
RRID | AB_2934962 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P22888 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LHCGR, also known as LHR or LSHR, belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 LHCGR antibody CL647-26424 | Download protocol |
FC protocol for CL Plus 647 LHCGR antibody CL647-26424 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |