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LHX8 Polyclonal antibody

LHX8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30722-1-AP

Print datasheet

Synonyms

LIM homeobox protein 8, LIM homeobox 8, Lhx7

验证数据展示

  • WB analysis using 30722-1-AP

    WB analysis using 30722-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30722-1-AP (LHX8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 30722-1-AP

    IF Staining of A549 using 30722-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LHX8 antibody (30722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30722-1-AP targets LHX8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen LHX8 fusion protein Ag33787 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LIM homeobox 8
Synonyms LIM homeobox protein 8, LIM homeobox 8, Lhx7
Calculated Molecular Weight 39 kDa
Observed Molecular Weight39-44 kDa
GenBank Accession NumberBC040321
Gene Symbol LHX8
Gene ID (NCBI) 431707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ68G74
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LHX8 (LIM homeobox 8), also known as LHX7. It is expected to be located in nucleus and mainly expressed in brain, lymphoid tissue, salivary gland. The calculated molecular weight of METAP1 is 39 kDa. The protein encoded by this gene is a member of the LIM homeobox family of proteins, which are involved in patterning and differentiation of various tissue types. These proteins contain two tandemly repeated cysteine-rich double-zinc finger motifs known as LIM domains, in addition to a DNA-binding homeodomain. It is also involved in oogenesis and in neuronal differentiation. LHX8 is a candidate gene for cleft palate, and it is also associated with odontoma formation (PMID: 34112263).

实验方案

Product Specific Protocols
WB protocol for LHX8 antibody 30722-1-APDownload protocol
IF protocol for LHX8 antibody 30722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols