验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human spleen tissue, human brain tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy LIG1 Ready-To-Use IHC Kit. LIG1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18051-1-AP targets DNA Ligase I in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | DNA Ligase I fusion protein Ag12508 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ligase I, DNA, ATP-dependent |
| 别名 | DNA ligase 1, DNA Ligase I, LIG1, ligase I, DNA, ATP dependent |
| 计算分子量 | 919 aa, 102 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC108318 |
| 基因名称 | LIG1 |
| Gene ID (NCBI) | 3978 |
| RRID | AB_2265726 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P18858 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 18051-1-AP is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNA Ligase I antibody 18051-1-AP | Download protocol |
| IHC protocol for DNA Ligase I antibody 18051-1-AP | Download protocol |
| IP protocol for DNA Ligase I antibody 18051-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma | ||
Nat Commun Cas9-induced large deletions and small indels are controlled in a convergent fashion. | ||
J Clin Invest Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.
| ||
Proc Natl Acad Sci U S A Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination.
| ||
Proc Natl Acad Sci U S A Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.
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