LIG3 Monoclonal antibody
LIG3 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B10B3
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HSC-T6 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy LIG3 Ready-To-Use IHC Kit. LIG3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68810-1-Ig targets LIG3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | LIG3 fusion protein Ag31484 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ligase III, DNA, ATP-dependent |
| Synonyms | DNA ligase 3, DNA ligase III, LIG2, LIG3, ligase III, DNA, ATP dependent |
| Calculated Molecular Weight | 100-110 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC068005 |
| Gene Symbol | LIG3 |
| Gene ID (NCBI) | 3980 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P49916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNA ligase 3 is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIG3 antibody 68810-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
