DNA ligase III/LIG3 Monoclonal antibody

DNA ligase III/LIG3 Monoclonal Antibody for WB, ELISA
Cat No. 68810-1-Ig

产品说明书

CloneNo. 1B10B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, ELISA

LIG3, LIG2, EC:6.5.1.1, DNA ligase III, DNA ligase 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy LIG3 Ready-To-Use IHC Kit. LIG3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68810-1-Ig targets DNA ligase III/LIG3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DNA ligase III/LIG3 fusion protein Ag31484 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ligase III, DNA, ATP-dependent
别名 LIG3, LIG2, EC:6.5.1.1, DNA ligase III, DNA ligase 3
计算分子量 100-110 kDa
观测分子量 100 kDa
GenBank蛋白编号BC068005
基因名称 LIG3
Gene ID (NCBI) 3980
RRIDAB_3670431
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49916
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA ligase III (DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.

实验方案

Product Specific Protocols
WB protocol for DNA ligase III/LIG3 antibody 68810-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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