DNA ligase III/LIG3 Monoclonal antibody, PBS Only (Capture)

DNA ligase III/LIG3 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10B3

Cat no : 68810-1-PBS

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Synonyms

LIG3, LIG2, EC:6.5.1.1, DNA ligase III, DNA ligase 3



Planning an IHC experiment? We recommend our IHCeasy LIG3 Ready-To-Use IHC Kit. LIG3 primary antibody included.

产品信息

68810-1-PBS targets DNA ligase III/LIG3 as part of a matched antibody pair:

MP50683-2: 68810-1-PBS capture and 68810-3-PBS detection (validated in Cytometric bead array)

MP50683-3: 68810-1-PBS capture and 68810-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DNA ligase III/LIG3 fusion protein Ag31484 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ligase III, DNA, ATP-dependent
Synonyms LIG3, LIG2, EC:6.5.1.1, DNA ligase III, DNA ligase 3
Calculated Molecular Weight 100-110 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC068005
Gene Symbol LIG3
Gene ID (NCBI) 3980
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49916
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA ligase III (DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.