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Species Reactivity
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验证数据展示

  • WB analysis of PC-3 using 66705-1-Ig

    WB analysis of PC-3 using 66705-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66705-1-Ig

    IF Staining of HepG2 using 66705-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66705-1-Ig (LIG4 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

LIG4 Monoclonal antibody

LIG4 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66705-1-Ig

Print datasheet

CloneNo. 1H6C11

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, HepG2 cells, Jurkat cells, Ramos cells, human testis tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66705-1-Ig targets LIG4 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen LIG4 fusion protein Ag3385 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ligase IV, DNA, ATP-dependent
Synonyms DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent
Calculated Molecular Weight 911 aa, 104 kDa
Observed Molecular Weight 100-104 kDa
GenBank Accession NumberBC037491
Gene Symbol LIG4
Gene ID (NCBI) 3981
RRIDAB_2882057
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49917
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.

实验方案

Product Specific Protocols
WB protocol for LIG4 antibody 66705-1-IgDownload protocol
IF protocol for LIG4 antibody 66705-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols