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验证数据展示

  • WB analysis of PC-3 using 66705-1-Ig

    WB analysis of PC-3 using 66705-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66705-1-Ig

    IF Staining of HepG2 using 66705-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66705-1-Ig (LIG4 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

LIG4 Monoclonal antibody

LIG4 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66705-1-Ig

产品说明书

CloneNo. 1H6C11

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, HepG2 cells, Jurkat cells, Ramos cells, human testis tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66705-1-Ig targets LIG4 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 LIG4 fusion protein Ag3385 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ligase IV, DNA, ATP-dependent
别名 DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent
计算分子量 911 aa, 104 kDa
观测分子量 100-104 kDa
GenBank蛋白编号BC037491
基因名称 LIG4
Gene ID (NCBI) 3981
RRIDAB_2882057
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49917
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.

实验方案

Product Specific Protocols
WB protocol for LIG4 antibody 66705-1-IgDownload protocol
IF protocol for LIG4 antibody 66705-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols