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LIG4 Monoclonal antibody, PBS Only

LIG4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H6C11

Cat no : 66705-1-PBS

Print datasheet

Synonyms

DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent

验证数据展示

  • WB analysis of PC-3 using 66705-1-Ig (same clone as 66705-1-PBS)

    WB analysis of PC-3 using 66705-1-Ig (same clone as 66705-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66705-1-Ig (same clone as 66705-1-PBS)

    IF Staining of HepG2 using 66705-1-Ig (same clone as 66705-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66705-1-Ig (LIG4 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

产品信息

66705-1-PBS targets LIG4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen LIG4 fusion protein Ag3385 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ligase IV, DNA, ATP-dependent
Synonyms DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent
Calculated Molecular Weight 911 aa, 104 kDa
Observed Molecular Weight 100-104 kDa
GenBank Accession NumberBC037491
Gene Symbol LIG4
Gene ID (NCBI) 3981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49917
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.