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验证数据展示

  • WB analysis using 14154-1-AP

    WB analysis using 14154-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14154-1-AP (LILRB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 14154-1-AP

    IF Staining of THP-1 using 14154-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using LILRB2 antibody (14154-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

LILRB2 Polyclonal antibody

LILRB2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14154-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Immunoglobulin-like transcript 4, ILT-4, ILT4, ILT 4, CD85d

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, THP-1 cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14154-1-AP targets LILRB2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 LILRB2 fusion protein Ag5336 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2
别名 Immunoglobulin-like transcript 4, ILT-4, ILT4, ILT 4, CD85d
计算分子量 598 aa, 65 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC036827
基因名称 LILRB2
Gene ID (NCBI) 10288
RRIDAB_3669180
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N423
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LILRB2 (Leukocyte immunoglobulin-like receptor subfamily B member 2), also known as ILT4, CD85d or MIR10, is a type I transmembrane glycoprotein that contains four extracellular immunoglobulin domains, a transmembrane domain, and three cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (PMID: 33928233). LILRB2 is mainly expressed in immunocytes including monocytes, macrophages, and dendritic cells, and transduces negative signals by its association with SHP-1 phosphatase via the ITIMs (PMID: 35717259). LILRB2 can interact with various ligands such as HLA-A, HLA-B, HLA-C, HLA-G, HLA-F, CD1c, CD1d, angiopoietin-like proteins (ANGPTLs), and SEMA4A.

实验方案

Product Specific Protocols
WB protocol for LILRB2 antibody 14154-1-APDownload protocol
IF protocol for LILRB2 antibody 14154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols