验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
20678-1-AP targets LIS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa |
Synonyms | LIS 1, LIS1, LIS2, Lissencephaly 1 protein, MDCR, MDS, PAF AH 45 kDa subunit, PAF AH alpha, PAFAH, PAFAH alpha, PAFAH1B1, PAFAHA |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 45-50 kDa |
GenBank Accession Number | NM_000430 |
Gene Symbol | LIS1 |
Gene ID (NCBI) | 5048 |
RRID | AB_2878723 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P43034 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet-activating factor acetylhydrolase 1B subunit alpha (LIS1), a critical mediator of neuronal migration in developing brain, is expressed throughout life. LIS1 forms a homodimer that interacts directly with the dynein complex and is essential for its enrichment at dynamic microtubule plus ends in mammalian cells. In addition to regulating the association of dynein with microtubule plus ends, LIS1 is required for efficient transport of many dynein-associated cargos and proper localization of Golgi complexes, endosomes, lysosomes, and mitochondria. The LIS1 protein has a calculated molecular mass of ~45 kDa.(PMID: 29150182, PMID: 29562183, PMID: 15800059)
实验方案
Product Specific Protocols | |
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WB protocol for LIS1 antibody 20678-1-AP | Download protocol |
IHC protocol for LIS1 antibody 20678-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |