• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60414-1-Ig

    WB analysis using 60414-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60414-1-Ig

    WB analysis using 60414-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 60414-1-Ig

    IHC staining of human colon using 60414-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60414-1-Ig (LMOD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 60414-1-Ig

    IF Staining of mouse heart using 60414-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using LMOD1 antibody (60414-1-Ig, Clone: 1D6H2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LMOD1 Monoclonal antibody

LMOD1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60414-1-Ig

产品说明书

CloneNo. 1D6H2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, ELISA

别名

Thyroid-associated ophthalmopathy autoantigen, SM-Lmod, Leiomodin-1, Leiomodin1, 1D6H2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inpig colon tissue, ARPE-19 cells, hTERT-RPE1 cells, rabbit colon tissue, rat colon tissue, mouse colon tissue, rabbit testis tissue, rat uterus tissue, mouse uterus tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60414-1-Ig targets LMOD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 LMOD1 fusion protein Ag7568 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 leiomodin 1 (smooth muscle)
别名 Thyroid-associated ophthalmopathy autoantigen, SM-Lmod, Leiomodin-1, Leiomodin1, 1D6H2
计算分子量 67 kDa
观测分子量 75 kDa
GenBank蛋白编号BC001755
基因名称 LMOD1
Gene ID (NCBI) 25802
RRIDAB_3670257
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP29536
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.

实验方案

Product Specific Protocols
WB protocol for LMOD1 antibody 60414-1-IgDownload protocol
IHC protocol for LMOD1 antibody 60414-1-IgDownload protocol
IF protocol for LMOD1 antibody 60414-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols