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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60414-1-Ig (same clone as 60414-1-PBS)

    WB analysis using 60414-1-Ig (same clone as 60414-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

  • WB analysis using 60414-1-Ig (same clone as 60414-1-PBS)

    WB analysis using 60414-1-Ig (same clone as 60414-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60414-1-Ig (LMOD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60414-1-Ig (same clone as 60414-1-PBS)

    IHC staining of human colon using 60414-1-Ig (same clone as 60414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60414-1-Ig (LMOD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 60414-1-Ig (same clone as 60414-1-PBS)

    IF Staining of mouse heart using 60414-1-Ig (same clone as 60414-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using LMOD1 antibody (60414-1-Ig, Clone: 1D6H2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60414-1-PBS in a different storage buffer formulation.

LMOD1 Monoclonal antibody, PBS Only

LMOD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60414-1-PBS
CloneNo. 1D6H2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Thyroid-associated ophthalmopathy autoantigen, SM-Lmod, Leiomodin-1, Leiomodin1, 1D6H2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

60414-1-PBS targets LMOD1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen LMOD1 fusion protein Ag7568 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name leiomodin 1 (smooth muscle)
Synonyms Thyroid-associated ophthalmopathy autoantigen, SM-Lmod, Leiomodin-1, Leiomodin1, 1D6H2
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC001755
Gene Symbol LMOD1
Gene ID (NCBI) 25802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP29536
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto's thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.