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验证数据展示

  • IF Staining of A431 using 83865-1-RR (same clone as 83865-1-PBS)

    IF Staining of A431 using 83865-1-RR (same clone as 83865-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using LOR antibody (83865-1-RR, Clone: 240605C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83865-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83865-1-RR (same clone as 83865-1-PBS)

    FC experiment of A431 using 83865-1-RR (same clone as 83865-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Loricrin Recombinant antibody (83865-1-RR, Clone:240605C8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83865-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skin using 83865-1-RR (same clone as 83865-1-PBS)

    IHC staining of mouse skin using 83865-1-RR (same clone as 83865-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83865-1-RR (Loricrin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83865-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skin using 83865-1-RR (same clone as 83865-1-PBS)

    IHC staining of mouse skin using 83865-1-RR (same clone as 83865-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83865-1-RR (Loricrin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83865-1-PBS in a different storage buffer formulation.

Loricrin Recombinant antibody, PBS Only

Loricrin Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83865-1-PBS

产品说明书

CloneNo. 240605C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

LOR, 240605C8, LRN

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83865-1-PBS targets Loricrin in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 loricrin
别名 LOR, 240605C8, LRN
计算分子量 26 kDa
GenBank蛋白编号NM_000427
基因名称 Loricrin
Gene ID (NCBI) 4014
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP23490
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Loricrin, also named LOR and LRN, is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. This antibody is specific to Loricrin.