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验证数据展示

  • IF Staining of A431 using 83865-1-RR

    IF Staining of A431 using 83865-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using LOR antibody (83865-1-RR, Clone: 240605C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 83865-1-RR

    FC experiment of A431 using 83865-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Loricrin Recombinant antibody (83865-1-RR, Clone:240605C8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of mouse skin using 83865-1-RR

    IHC staining of mouse skin using 83865-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83865-1-RR (Loricrin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 83865-1-RR

    IHC staining of mouse skin using 83865-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83865-1-RR (Loricrin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Loricrin Recombinant antibody

Loricrin Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83865-1-RR

产品说明书

CloneNo. 240605C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

LOR, 240605C8, LRN

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83865-1-RR targets Loricrin in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 loricrin
别名 LOR, 240605C8, LRN
计算分子量 26 kDa
GenBank蛋白编号NM_000427
基因名称 Loricrin
Gene ID (NCBI) 4014
RRIDAB_3671451
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP23490
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Loricrin, also named LOR and LRN, is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. This antibody is specific to Loricrin.

实验方案

Product Specific Protocols
IHC protocol for Loricrin antibody 83865-1-RRDownload protocol
IF protocol for Loricrin antibody 83865-1-RRDownload protocol
FC protocol for Loricrin antibody 83865-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols