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验证数据展示

  • IF Staining of HeLa using CL594-66044

    IF Staining of HeLa using CL594-66044

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66044 (LPCAT1 antibody) at dilution of 1:100.

  • IF Staining of HeLa using CL594-66044

    IF Staining of HeLa using CL594-66044

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 LPCAT1 antibody (CL594-66044, Clone: 8B6E9 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated LPCAT1 Monoclonal antibody

LPCAT1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66044

Print datasheet

CloneNo. 8B6E9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Acetyl-CoA:lyso-PAF acetyltransferase, 8B6E9, 1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LPCAT1 Ready-To-Use IHC Kit. LPCAT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66044-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66044 targets LPCAT1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen LPCAT1 fusion protein Ag9060 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name lysophosphatidylcholine acyltransferase 1
Synonyms Acetyl-CoA:lyso-PAF acetyltransferase, 8B6E9, 1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase
Calculated Molecular Weight 534 aa, 59 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC020166
Gene Symbol LPCAT1
Gene ID (NCBI) 79888
RRIDAB_2883489
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NF37
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).

实验方案

Product Specific Protocols
IF protocol for CL594 LPCAT1 antibody CL594-66044Download protocol
Standard Protocols
Click here to view our Standard Protocols