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验证数据展示

  • IF Staining of HeLa using CL594-66044

    IF Staining of HeLa using CL594-66044

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66044 (LPCAT1 antibody) at dilution of 1:100.

  • IF Staining of HeLa using CL594-66044

    IF Staining of HeLa using CL594-66044

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 LPCAT1 antibody (CL594-66044, Clone: 8B6E9 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated LPCAT1 Monoclonal antibody

LPCAT1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66044

产品说明书

CloneNo. 8B6E9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Acetyl-CoA:lyso-PAF acetyltransferase, 8B6E9, 1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LPCAT1 Ready-To-Use IHC Kit. LPCAT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66044-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66044 targets LPCAT1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 LPCAT1 fusion protein Ag9060 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 lysophosphatidylcholine acyltransferase 1
别名 Acetyl-CoA:lyso-PAF acetyltransferase, 8B6E9, 1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase
计算分子量 534 aa, 59 kDa
观测分子量 55 kDa
GenBank蛋白编号BC020166
基因名称 LPCAT1
Gene ID (NCBI) 79888
RRIDAB_2883489
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NF37
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).

实验方案

Product Specific Protocols
IF protocol for CL594 LPCAT1 antibody CL594-66044Download protocol
Standard Protocols
Click here to view our Standard Protocols