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LPCAT2 Polyclonal antibody

LPCAT2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15082-1-AP

Print datasheet

Synonyms

1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-AGPAT 11, 1-AGP acyltransferase 11, 1-acylglycerophosphocholine O-acyltransferase

验证数据展示

  • WB analysis using 15082-1-AP

    WB analysis using 15082-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 15082-1-AP

    IF Staining of A431 using 15082-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using LPCAT2 antibody (15082-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, LPS treated HEK-293, HeLa cells, Jurkat cells, RAW 264.7 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy LPCAT2 Ready-To-Use IHC Kit. LPCAT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15082-1-AP targets LPCAT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LPCAT2 fusion protein Ag7156 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysophosphatidylcholine acyltransferase 2
Synonyms 1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-AGPAT 11, 1-AGP acyltransferase 11, 1-acylglycerophosphocholine O-acyltransferase
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC002472
Gene Symbol LPCAT2
Gene ID (NCBI) 54947
RRIDAB_2135557
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L5N7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LPCAT2 antibody 15082-1-APDownload protocol
IF protocol for LPCAT2 antibody 15082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Antioxidants (Basel)

Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis

Authors - Melanie Merkel
View Article