验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, LPS treated HEK-293, HeLa cells, Jurkat cells, RAW 264.7 cells |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy LPCAT2 Ready-To-Use IHC Kit. LPCAT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
15082-1-AP targets LPCAT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | LPCAT2 fusion protein Ag7156 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lysophosphatidylcholine acyltransferase 2 |
| 别名 | 1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-AGPAT 11, 1-AGP acyltransferase 11, 1-acylglycerophosphocholine O-acyltransferase |
| 计算分子量 | 60 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC002472 |
| 基因名称 | LPCAT2 |
| Gene ID (NCBI) | 54947 |
| RRID | AB_2135557 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7L5N7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LPCAT2 antibody 15082-1-AP | Download protocol |
| IF protocol for LPCAT2 antibody 15082-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
