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验证数据展示

  • IF Staining of NIH/3T3 using CL488-68124

    IF Staining of NIH/3T3 using CL488-68124

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 LPIN1 antibody (CL488-68124, Clone: 3G11B10 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated LPIN1 Monoclonal antibody

LPIN1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68124

产品说明书

CloneNo. 3G11B10

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Rat, Mouse

应用

IF/ICC

别名

DKFZp781P1796, KIAA0188, lipin 1, LPIN1, PAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy LPIN1 Ready-To-Use IHC Kit. LPIN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68124-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68124 targets LPIN1 in IF/ICC applications and shows reactivity with Human, Rat, Mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Rat, Mouse
免疫原 LPIN1 fusion protein Ag25813 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 lipin 1
别名 DKFZp781P1796, KIAA0188, lipin 1, LPIN1, PAP1
计算分子量 890 aa, 99 kDa
观测分子量130 kDa
GenBank蛋白编号BC030537
基因名称 LPIN1
Gene ID (NCBI) 23175
RRIDAB_3084407
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14693
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPIN1, also named as KIAA0188, belongs to the lipin family.It is a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. LPIN1 has a molecular weight of about 99 kDa but predicted bands around 130 kDa.(PMID:20231281, PMID:29765047).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LPIN1 antibody CL488-68124Download protocol
Standard Protocols
Click here to view our Standard Protocols