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CoraLite® Plus 488-conjugated LPIN1 Monoclonal antibody

LPIN1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G11B10

Cat no : CL488-68124

Print datasheet

Synonyms

DKFZp781P1796, KIAA0188, lipin 1, LPIN1, PAP1

验证数据展示

  • IF Staining of NIH/3T3 using CL488-68124

    IF Staining of NIH/3T3 using CL488-68124

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 LPIN1 antibody (CL488-68124, Clone: 3G11B10 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy LPIN1 Ready-To-Use IHC Kit. LPIN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68124-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68124 targets LPIN1 in IF/ICC applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen LPIN1 fusion protein Ag25813 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name lipin 1
Synonyms DKFZp781P1796, KIAA0188, lipin 1, LPIN1, PAP1
Calculated Molecular Weight 890 aa, 99 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC030537
Gene Symbol LPIN1
Gene ID (NCBI) 23175
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14693
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPIN1, also named as KIAA0188, belongs to the lipin family.It is a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. LPIN1 has a molecular weight of about 99 kDa but predicted bands around 130 kDa.(PMID:20231281, PMID:29765047).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LPIN1 antibody CL488-68124Download protocol
Standard Protocols
Click here to view our Standard Protocols