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LPXN Polyclonal antibody
LPXN Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IF/ICC detected in | PC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy LPXN Ready-To-Use IHC Kit. LPXN primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
11307-1-AP targets LPXN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Immunogen | LPXN fusion protein Ag1845 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | leupaxin |
| Synonyms | LDPL, LDLP |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC019035 |
| Gene Symbol | LPXN |
| Gene ID (NCBI) | 9404 |
| RRID | AB_2877756 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60711 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LPXN antibody 11307-1-AP | Download protocol |
| IF protocol for LPXN antibody 11307-1-AP | Download protocol |
| IP protocol for LPXN antibody 11307-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Mol Cell Cardiol Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG. | ||