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LPXN Recombinant antibody, PBS Only

LPXN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240483G10

Cat no : 83657-5-PBS

Print datasheet

Synonyms

LDPL, LDLP, 240483G10

验证数据展示

  • WB analysis using 83657-5-RR (same clone as 83657-5-PBS)

    WB analysis using 83657-5-RR (same clone as 83657-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83657-5-RR (LPXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83657-5-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 83657-5-RR (same clone as 83657-5-PBS)

    IF Staining of PC-3 using 83657-5-RR (same clone as 83657-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using LPXN antibody (83657-5-RR, Clone: 240483G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83657-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83657-5-RR

    Affinity and Kinetic Characterization of 83657-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83657-5-RR against Human LPXN were performed. The affinity constant is 0.187 nM.

Planning an IHC experiment? We recommend our IHCeasy LPXN Ready-To-Use IHC Kit. LPXN primary antibody included.

产品信息

The immunogen of 83657-5-PBS is LPXN Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen LPXN fusion protein Ag34956 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name leupaxin
Synonyms LDPL, LDLP, 240483G10
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC019035
Gene Symbol LPXN
Gene ID (NCBI) 9404
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60711
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).