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LPXN Recombinant antibody

LPXN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240483G10

Cat no : 83657-5-RR

Print datasheet

Synonyms

LDPL, LDLP, 240483G10

验证数据展示

  • WB analysis using 83657-5-RR

    WB analysis using 83657-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83657-5-RR (LPXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 83657-5-RR

    IF Staining of PC-3 using 83657-5-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using LPXN antibody (83657-5-RR, Clone: 240483G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83657-5-RR

    Affinity and Kinetic Characterization of 83657-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83657-5-RR against Human LPXN were performed. The affinity constant is 0.187 nM.

经过测试的应用

Positive WB detected inRamos cells, Jurkat cells, MDA-MB-231 cells
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy LPXN Ready-To-Use IHC Kit. LPXN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83657-5-RR is LPXN Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen LPXN fusion protein Ag34956 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name leupaxin
Synonyms LDPL, LDLP, 240483G10
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC019035
Gene Symbol LPXN
Gene ID (NCBI) 9404
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60711
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).

实验方案

Product Specific Protocols
WB protocol for LPXN antibody 83657-5-RRDownload protocol
IF protocol for LPXN antibody 83657-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols