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LRP2-Specific Polyclonal antibody
LRP2-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human brain tissue, human placenta tissue, HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human kidney tissue |
Planning an IHC experiment? We recommend our IHCeasy LRP2 Ready-To-Use IHC Kit. LRP2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19700-1-AP targets LRP2-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | low density lipoprotein-related protein 2 |
| Synonyms | DBS, Fx1a, Glycoprotein 330, gp330, LRP 2, LRP2, LRP2-Specific, Megalin |
| Calculated Molecular Weight | 522 kDa |
| Observed Molecular Weight | 280 kDa |
| GenBank Accession Number | NM_004525 |
| Gene Symbol | LRP2 |
| Gene ID (NCBI) | 4036 |
| RRID | AB_10640428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98164 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRP2, also named as gp330 and Megalin, belongs to the LDLR family. It acts together with cubilin to mediate HDL endocytosis. LRP2 may participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS). This antibody is specific to LRP2 and detects endogenous LRP2 with an apparent molecular weight 280 kDa, which is smaller than the predicted molecular weight (522 kDa) and probably represent a proteolytic fragment of the large glycoprotein (PMID: 16638803; 8483924).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LRP2-Specific antibody 19700-1-AP | Download protocol |
| IHC protocol for LRP2-Specific antibody 19700-1-AP | Download protocol |
| IF protocol for LRP2-Specific antibody 19700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance. | ||
Nephrol Dial Transplant Reduced proximal tubular expression of protein endocytic receptors in proteinuria is associated with urinary receptor shedding. | ||
Development Mouse early extra-embryonic lineages activate compensatory endocytosis in response to poor maternal nutrition. | ||
Metabolism Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy. | ||
Mol Ther Nucleic Acids Gelofusine Attenuates Tubulointerstitial Injury Induced by cRGD-Conjugated siRNA by Regulating the TLR3 Signaling Pathway. | ||
Stem Cell Res Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system.
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