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  • Featured Product
  • KD/KO Validated

LRPPRC Polyclonal antibody

LRPPRC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 21175-1-AP

Print datasheet

Synonyms

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 130 kDa leucine-rich protein

验证数据展示

  • WB analysis using 21175-1-AP

    WB analysis using 21175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 21175-1-AP

    IF Staining of U2OS using 21175-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 21175-1-AP (LRPPRC antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

  • IHC staining of human normal colon using 21175-1-AP

    IHC staining of human normal colon using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human kidney using 21175-1-AP

    IHC staining of human kidney using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human kidney using 21175-1-AP

    IHC staining of human kidney using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • WB analysis using 21175-1-AP

    WB analysis using 21175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human normal colon using 21175-1-AP

    IHC staining of human normal colon using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IP experiment of HeLa using 21175-1-AP

    IP experiment of HeLa using 21175-1-AP

    IP result of anti-LRPPRC (IP:21175-1-AP, 5ug; Detection:21175-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • IF Staining of HEK-293 using 21175-1-AP

    IF Staining of HEK-293 using 21175-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (21175-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHCT 116 cells, mouse heart tissue, HepG2 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, HeLa cells, rat kidney tissue, rat liver tissue, rat heart tissue, PC-12 cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human heart tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HepG2 cells, HeLa cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy LRPPRC Ready-To-Use IHC Kit. LRPPRC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21175-1-AP targets LRPPRC in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen LRPPRC fusion protein Ag15452 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine-rich PPR-motif containing
Synonyms LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 130 kDa leucine-rich protein
Calculated Molecular Weight 1394 aa, 158 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC050311
Gene Symbol LRPPRC
Gene ID (NCBI) 10128
RRIDAB_10733879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42704
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

实验方案

Product Specific Protocols
WB protocol for LRPPRC antibody 21175-1-APDownload protocol
IHC protocol for LRPPRC antibody 21175-1-APDownload protocol
IF protocol for LRPPRC antibody 21175-1-APDownload protocol
IP protocol for LRPPRC antibody 21175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIP

Nat Biotechnol

Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.

Authors - Xue-Hai Liang
View Article
mouseIF,WB,CoIP

Nat Commun

Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA

Authors - Ayesha Sen
View Article
humanWB,IF

Nat Commun

A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.

Authors - Peng Sun
View Article
humanWB

Genome Biol

Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes

Authors - Iliana Soto
  • KO Validated
View Article
humanIHC,CoIP

Redox Biol

LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.

Authors - Wen-Su Wei
View Article
humanWB

EMBO J

Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.

Authors - Yanni Ma
View Article