验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, mouse heart tissue, HepG2 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, HeLa cells, rat kidney tissue, rat liver tissue, rat heart tissue, PC-12 cells, SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human heart tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, HepG2 cells, HeLa cells, HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21175-1-AP targets LRPPRC in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | LRPPRC fusion protein Ag15452 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leucine-rich PPR-motif containing |
| 别名 | LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 130 kDa leucine-rich protein |
| 计算分子量 | 1394 aa, 158 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC050311 |
| 基因名称 | LRPPRC |
| Gene ID (NCBI) | 10128 |
| RRID | AB_10733879 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42704 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| IHC protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| IF protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| IP protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames. | ||
Nat Commun Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA | ||
Nat Commun A mosquito salivary protein promotes flavivirus transmission by activation of autophagy. | ||
Genome Biol Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes
| ||
Redox Biol LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis. | ||
EMBO J Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells. |
