- Featured Product
- KD/KO Validated
LRPPRC Polyclonal antibody
LRPPRC Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, mouse heart tissue, HepG2 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, HeLa cells, rat kidney tissue, rat liver tissue, rat heart tissue, PC-12 cells, SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | U2OS cells, HepG2 cells, HeLa cells, HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy LRPPRC Ready-To-Use IHC Kit. LRPPRC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21175-1-AP targets LRPPRC in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA Application Description |
| Cited Applications | WB, IP, IF, RIP, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | LRPPRC fusion protein Ag15452 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | leucine-rich PPR-motif containing |
| Synonyms | 130 kDa leucine rich protein, CLONE 23970, GP130, LRP 130, LRP130, LRPPRC, LSFC |
| Calculated Molecular Weight | 1394 aa, 158 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC050311 |
| Gene Symbol | LRPPRC |
| Gene ID (NCBI) | 10128 |
| RRID | AB_10733879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42704 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| IHC protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| IF protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| IP protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames. | ||
Nat Commun Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA | ||
Nat Commun A mosquito salivary protein promotes flavivirus transmission by activation of autophagy. | ||
Genome Biol Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes
| ||
Redox Biol LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis. | ||
EMBO J Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells. |
