验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy LRPPRC Ready-To-Use IHC Kit. LRPPRC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21175-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-21175 targets LRPPRC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | LRPPRC fusion protein Ag15452 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leucine-rich PPR-motif containing |
| 别名 | LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 130 kDa leucine-rich protein |
| 计算分子量 | 1394 aa, 158 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC050311 |
| 基因名称 | LRPPRC |
| Gene ID (NCBI) | 10128 |
| RRID | AB_3084049 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42704 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LRPPRC antibody CL488-21175 | Download protocol |
| FC protocol for CL Plus 488 LRPPRC antibody CL488-21175 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |