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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated LRPPRC Polyclonal antibody

LRPPRC Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21175

Print datasheet

Synonyms

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 130 kDa leucine-rich protein

验证数据展示

  • FC experiment of HEK-293 using CL488-21175

    FC experiment of HEK-293 using CL488-21175

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human LRPPRC (CL488-21175)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HEK-293 using CL488-21175

    IF Staining of HEK-293 using CL488-21175

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 LRPPRC antibody (CL488-21175) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy LRPPRC Ready-To-Use IHC Kit. LRPPRC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21175-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21175 targets LRPPRC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen LRPPRC fusion protein Ag15452 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine-rich PPR-motif containing
Synonyms LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 130 kDa leucine-rich protein
Calculated Molecular Weight 1394 aa, 158 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC050311
Gene Symbol LRPPRC
Gene ID (NCBI) 10128
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42704
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LRPPRC antibody CL488-21175Download protocol
FC protocol for CL Plus 488 LRPPRC antibody CL488-21175Download protocol
Standard Protocols
Click here to view our Standard Protocols