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验证数据展示

  • WB analysis using 67679-1-Ig (same clone as 67679-1-PBS)

    WB analysis using 67679-1-Ig (same clone as 67679-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67679-1-Ig (same clone as 67679-1-PBS)

    IHC staining of human colon cancer using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human colon cancer using 67679-1-Ig (same clone as 67679-1-PBS)

    IHC staining of human colon cancer using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IF Staining of HepG2 using 67679-1-Ig (same clone as 67679-1-PBS)

    IF Staining of HepG2 using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67679-1-Ig (same clone as 67679-1-PBS)

    IF Staining of HeLa using 67679-1-Ig (same clone as 67679-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67679-1-Ig (same clone as 67679-1-PBS)

    WB analysis of HeLa using 67679-1-Ig (same clone as 67679-1-PBS)

    WB result of LRPPRC antibody (67679-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LRPPRC transfected HeLa cells. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

LRPPRC Monoclonal antibody, PBS Only

LRPPRC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67679-1-PBS

产品说明书

CloneNo. 3H7E1

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 3H7E1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy LRPPRC Ready-To-Use IHC Kit. LRPPRC primary antibody included.

产品信息

67679-1-PBS targets LRPPRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 LRPPRC fusion protein Ag30261 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 leucine-rich PPR-motif containing
别名 LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 3H7E1
计算分子量 1394 aa, 158 kDa
观测分子量130 kDa
GenBank蛋白编号BC050311
基因名称 LRPPRC
Gene ID (NCBI) 10128
RRIDAB_2882872
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42704
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.