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  • Featured Product
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LRPPRC Monoclonal antibody

LRPPRC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3H7E1

Cat no : 67679-1-Ig

Print datasheet

Synonyms

LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 3H7E1

验证数据展示

  • WB analysis using 67679-1-Ig

    WB analysis using 67679-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67679-1-Ig

    IHC staining of human colon cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human colon cancer using 67679-1-Ig

    IHC staining of human colon cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IF Staining of HepG2 using 67679-1-Ig

    IF Staining of HepG2 using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • IF Staining of HeLa using 67679-1-Ig

    IF Staining of HeLa using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 67679-1-Ig

    WB analysis of HeLa using 67679-1-Ig

    WB result of LRPPRC antibody (67679-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LRPPRC transfected HeLa cells.

经过测试的应用

Positive WB detected inU2OS cells, U-251 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human endometrial cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells, HepG2 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy LRPPRC Ready-To-Use IHC Kit. LRPPRC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67679-1-Ig targets LRPPRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen LRPPRC fusion protein Ag30261 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name leucine-rich PPR-motif containing
Synonyms LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 3H7E1
Calculated Molecular Weight 1394 aa, 158 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC050311
Gene Symbol LRPPRC
Gene ID (NCBI) 10128
RRIDAB_2882872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42704
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

实验方案

Product Specific Protocols
WB protocol for LRPPRC antibody 67679-1-IgDownload protocol
IHC protocol for LRPPRC antibody 67679-1-IgDownload protocol
IF protocol for LRPPRC antibody 67679-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Front Immunol

Expression and clinical significance of interleukin-6 pathway in cholangiocarcinoma

Authors - Dongqing Gu
View Article
humanWB

Front Genet

RNA methylation pattern and immune microenvironment characteristics mediated by m6A regulator in ischemic stroke

Authors - Kejuan Jia
View Article