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- KD/KO Validated
CoraLite®594-conjugated LRPPRC Monoclonal antibody
LRPPRC Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3H7E1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy LRPPRC Ready-To-Use IHC Kit. LRPPRC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67679-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67679 targets LRPPRC in IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | LRPPRC fusion protein Ag30261 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | leucine-rich PPR-motif containing |
| Synonyms | LRP130, LRP 130, Leucine-rich PPR motif-containing protein, mitochondrial, GP130, 3H7E1 |
| Calculated Molecular Weight | 1394 aa, 158 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC050311 |
| Gene Symbol | LRPPRC |
| Gene ID (NCBI) | 10128 |
| RRID | AB_2920170 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42704 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 LRPPRC antibody CL594-67679 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |