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验证数据展示

  • WB analysis using 83693-3-RR

    WB analysis using 83693-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83693-3-RR (LRRC47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 83693-3-RR

    IF Staining of L02 using 83693-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using LRRC47 antibody (83693-3-RR, Clone: 240741A6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of U2OS using 83693-3-RR

    FC experiment of U2OS using 83693-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug LRRC47 Recombinant antibody (83693-3-RR, Clone:240741A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83693-3-RR

    Affinity and Kinetic Characterization of 83693-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83693-3-RR against Human LRRC47 were performed. The affinity constant is below 1 pM.

LRRC47 Recombinant antibody

LRRC47 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83693-3-RR

产品说明书

CloneNo. 240741A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Leucine-rich repeat-containing protein 47, KIAA1185, 240741A6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, U-87 MG cells, L02 cells
Positive IF/ICC detected inL02 cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83693-3-RR targets LRRC47 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 LRRC47 fusion protein Ag19736 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leucine rich repeat containing 47
别名 Leucine-rich repeat-containing protein 47, KIAA1185, 240741A6
计算分子量 583 aa, 63 kDa
观测分子量63 kDa
GenBank蛋白编号BC031301
基因名称 LRRC47
Gene ID (NCBI) 57470
RRIDAB_3671296
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8N1G4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.

实验方案

Product Specific Protocols
WB protocol for LRRC47 antibody 83693-3-RRDownload protocol
IF protocol for LRRC47 antibody 83693-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols