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LRRC47 Recombinant antibody

LRRC47 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240741A6

Cat no : 83693-3-RR

Print datasheet

Synonyms

Leucine-rich repeat-containing protein 47, KIAA1185, 240741A6

验证数据展示

  • WB analysis using 83693-3-RR

    WB analysis using 83693-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83693-3-RR (LRRC47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 83693-3-RR

    IF Staining of L02 using 83693-3-RR

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using LRRC47 antibody (83693-3-RR, Clone: 240741A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of U2OS using 83693-3-RR

    FC experiment of U2OS using 83693-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug LRRC47 Recombinant antibody (83693-3-RR, Clone:240741A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83693-3-RR

    Affinity and Kinetic Characterization of 83693-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83693-3-RR against Human LRRC47 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inDaudi cells, U-87 MG cells, L02 cells
Positive IF/ICC detected inL02 cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83693-3-RR is LRRC47 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen LRRC47 fusion protein Ag19736 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name leucine rich repeat containing 47
Synonyms Leucine-rich repeat-containing protein 47, KIAA1185, 240741A6
Calculated Molecular Weight 583 aa, 63 kDa
Observed Molecular Weight63 kDa
GenBank Accession NumberBC031301
Gene Symbol LRRC47
Gene ID (NCBI) 57470
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8N1G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.

实验方案

Product Specific Protocols
WB protocol for LRRC47 antibody 83693-3-RRDownload protocol
IF protocol for LRRC47 antibody 83693-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols