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验证数据展示

  • WB analysis using 68323-1-Ig

    WB analysis using 68323-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68323-1-Ig (LRRC8C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68323-1-Ig

    IF Staining of HepG2 using 68323-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LRRC8C antibody (68323-1-Ig, Clone: 4A6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

LRRC8C Monoclonal antibody

LRRC8C Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68323-1-Ig

产品说明书

CloneNo. 4A6A4

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

AD158, DKFZp586J1119, FAD158, LRRC8C

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, HEK-239 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68323-1-Ig targets LRRC8C in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 LRRC8C fusion protein Ag17001 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 leucine rich repeat containing 8 family, member C
别名 AD158, DKFZp586J1119, FAD158, LRRC8C
计算分子量 803 aa, 92 kDa
观测分子量105 kDa
GenBank蛋白编号BC113973
基因名称 LRRC8C
Gene ID (NCBI) 84230
RRIDAB_2935395
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TDW0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRRC8C (Leucine-rich repeat-containing protein 8C), also known as AD158, is a non-essential component of the volume-regulated anion channel (VRAC, also known as VSOAC channel), which is required to maintain a constant cell volume in response to extracellular or intracellular permeability changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. The VRAC channel also mediates transport of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol (PMID:24790029; 26824658; 28193731).

实验方案

Product Specific Protocols
WB protocol for LRRC8C antibody 68323-1-IgDownload protocol
IF protocol for LRRC8C antibody 68323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols