• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84778-2-RR (same clone as 84778-2-PBS)

    WB analysis using 84778-2-RR (same clone as 84778-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-2-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01600-1

    Sandwich ELISA standard curve of MP01600-1

    Sandwich ELISA standard curve of MP01600-1, Human LRRFIP1 Recombinant Matched Antibody Pair - PBS only. 84778-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34760. 84778-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-2500 pg/mL

  • Sample test of MP01600-1

    Sample test of MP01600-1

    The mean LRRFIP1 concentration was determined to be 146.23 ng/mL in Raji cell extract based on a 1.4 mg/mL extract load, 70.95 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 131.01 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP01600-1

    Cytometric bead array standard curve of MP01600-1

    Cytometric bead array standard curve of MP01600-1, LRRFIP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84778-1-PBS. Detection antibody: 84778-2-PBS. Standard: Ag34760. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01600-2

    Cytometric bead array standard curve of MP01600-2

    Cytometric bead array standard curve of MP01600-2, LRRFIP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84778-3-PBS. Detection antibody: 84778-2-PBS. Standard: Ag34760. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84778-2-RR

    Affinity and Kinetic Characterization of 84778-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84778-2-RR against Human LRRFIP1 were performed. The affinity constant is below 1 pM.

LRRFIP1 Recombinant antibody, PBS Only (Detector)

LRRFIP1 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84778-2-PBS

产品说明书

CloneNo. 242161F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1, FLAP 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84778-2-PBS targets LRRFIP1 as part of a matched antibody pair:

MP01600-1: 84778-1-PBS capture and 84778-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01600-2: 84778-3-PBS capture and 84778-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 LRRFIP1 fusion protein Ag34760 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leucine rich repeat (in FLII) interacting protein 1
别名 GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1, FLAP 1
观测分子量150-170 kDa
GenBank蛋白编号BC108914
基因名称 LRRFIP1
Gene ID (NCBI) 9208
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ32MZ4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).