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Host
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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

    WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

  • WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

    WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

  • WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

    WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84778-4-RR (same clone as 84778-4-PBS)

    IF Staining of A431 using 84778-4-RR (same clone as 84778-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using LRRFIP1 antibody (84778-4-RR, Clone: 242161C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

LRRFIP1 Recombinant antibody, PBS Only

LRRFIP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84778-4-PBS

产品说明书

CloneNo. 242161C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1, FLAP 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84778-4-PBS targets LRRFIP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 LRRFIP1 fusion protein Ag34760 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leucine rich repeat (in FLII) interacting protein 1
别名 GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1, FLAP 1
观测分子量170 kDa
GenBank蛋白编号BC108914
基因名称 LRRFIP1
Gene ID (NCBI) 9208
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ32MZ4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).