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LRRFIP1 Recombinant antibody

LRRFIP1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242161C9

Cat no : 84778-4-RR

Print datasheet

Synonyms

GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1, FLAP 1

验证数据展示

  • WB analysis using 84778-4-RR

    WB analysis using 84778-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84778-4-RR

    WB analysis using 84778-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84778-4-RR

    WB analysis using 84778-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84778-4-RR

    IF Staining of A431 using 84778-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using LRRFIP1 antibody (84778-4-RR, Clone: 242161C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA549 cells, HL-60 cells, Raji cells, COLO 320 cells, K-562 cells, HEK-293 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84778-4-RR targets LRRFIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen LRRFIP1 fusion protein Ag34760 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name leucine rich repeat (in FLII) interacting protein 1
Synonyms GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1, FLAP 1
Observed Molecular Weight170 kDa
GenBank Accession NumberBC108914
Gene Symbol LRRFIP1
Gene ID (NCBI) 9208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ32MZ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

实验方案

Product Specific Protocols
WB protocol for LRRFIP1 antibody 84778-4-RRDownload protocol
IF protocol for LRRFIP1 antibody 84778-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols