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LRWD1 Polyclonal antibody

LRWD1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 19546-1-AP

Print datasheet

Synonyms

LRWD1

验证数据展示

  • WB analysis using 19546-1-AP

    WB analysis using 19546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19546-1-AP (LRWD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 19546-1-AP

    IP experiment of mouse testis using 19546-1-AP

    IP result of anti-LRWD1 (IP:19546-1-AP, 4ug; Detection:19546-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19546-1-AP targets LRWD1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine-rich repeats and WD repeat domain containing 1
Synonyms LRWD1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberNM_152892
Gene Symbol LRWD1
Gene ID (NCBI) 222229
RRIDAB_2878588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UFC0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRWD1 contains 3 LRR (leucine-rich) repeats and 5 WD repeats. LRWD1 is phosphorylated upon DNA damage by ATM or ATR. The antibody recognize the N-term of LRWD1.

实验方案

Product Specific Protocols
WB protocol for LRWD1 antibody 19546-1-APDownload protocol
IP protocol for LRWD1 antibody 19546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Development

PRSS50 is a testis protease responsible for proper sperm tail formation and function.

Authors - Jason M Scovell
View Article