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验证数据展示

  • FC experiment of HeLa using CL488-67029

    FC experiment of HeLa using CL488-67029

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated LSM12 Monoclonal antibody (CL488-67029, Clone:2E3G3)(red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated LSM12 Monoclonal antibody

LSM12 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67029

产品说明书

CloneNo. 2E3G3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

Protein LSM12, 2E3G3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67029-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67029 targets LSM12 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 LSM12 fusion protein Ag28782 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 LSM12 homolog (S. cerevisiae)
别名 Protein LSM12, 2E3G3
观测分子量 22 kDa
GenBank蛋白编号BC044587
基因名称 LSM12
Gene ID (NCBI) 124801
RRIDAB_2934514
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ3MHD2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 LSM12 antibody CL488-67029Download protocol
Standard Protocols
Click here to view our Standard Protocols