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验证数据展示

  • IP experiment of U2OS using 28824-1-AP

    IP experiment of U2OS using 28824-1-AP

    IP result of anti-LTBP2 (IP:28824-1-AP, 4ug; Detection:28824-1-AP 1:600) with U2OS cells lysate 1840 ug.

  • IHC staining of mouse lung using 28824-1-AP

    IHC staining of mouse lung using 28824-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28824-1-AP (LTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 28824-1-AP

    IHC staining of mouse lung using 28824-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28824-1-AP (LTBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung using 28824-1-AP

    IHC staining of human lung using 28824-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28824-1-AP (LTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 28824-1-AP

    IF Staining of U2OS using 28824-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using LTBP2 antibody (28824-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

LTBP2 Polyclonal antibody

LTBP2 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 28824-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, IP, ELISA

别名

C14orf141, LTBP3, Latent-transforming growth factor beta-binding protein 2, LTBP 2, LTBP-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inU2OS cells
Positive IHC detected inmouse lung tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28824-1-AP targets LTBP2 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 LTBP2 fusion protein Ag29737 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 latent transforming growth factor beta binding protein 2
别名 C14orf141, LTBP3, Latent-transforming growth factor beta-binding protein 2, LTBP 2, LTBP-2
计算分子量 1821 aa, 195 kDa
观测分子量 260 kDa
GenBank蛋白编号BC078659
基因名称 LTBP2
Gene ID (NCBI) 4053
RRIDAB_3669670
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14767
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LTBP2, an extracellular glycoprotein mapping to chromosome 14q24, belongs to a family of latent TGF-β binding proteins (LTBPs) that regulates a well-known growth factor, TGF-β. LTBP2 is an elastic fiber-associating protein whose function in elastogenesis is not clear, as a DANCEbinding Protein. (PMID: 17581631 )It may play an integral structural role in elastic-fiber architectural organization and/or assembly. LTBP2 associates covalently with small latent TGF-beta complex via Repeat B and Repeat C. Defects in LTBP2 are the cause of primary congenital glaucoma type 3D (GLC3D). [MIM:613086] LTBP2 is a role in the structural stability of ciliary zonules, and growth and development of lens.(PMID: 20617341) LTBP2 null mutations in an autosomal recessive ocular syndrome with megalocornea, spherophakia, and secondary glaucoma (PMID: 20179738 ) . LTBP2 is a biomarker of heart failure. LTBP2 has a molecular weight of 260 kDa (PMID: 20878956), and even larger molecular weights of 290 and 310 kDa were detected in tissue extracts. (PMID: 8524260)

实验方案

Product Specific Protocols
IHC protocol for LTBP2 antibody 28824-1-APDownload protocol
IF protocol for LTBP2 antibody 28824-1-APDownload protocol
IP protocol for LTBP2 antibody 28824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols