CoraLite® Plus 488-conjugated LYN Monoclonal antibody
LYN Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C7F2
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Ramos cells |
Planning an IHC experiment? We recommend our IHCeasy LYN Ready-To-Use IHC Kit. LYN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60211-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60211 targets LYN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | LYN fusion protein Ag12723 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-yes-1 Yamaguchi sarcoma viral related oncogene homolog |
| Synonyms | Tyrosine-protein kinase Lyn, Tyrosine protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, JTK8, EC:2.7.10.2 |
| Calculated Molecular Weight | 512 aa, 59 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC075002 |
| Gene Symbol | LYN |
| Gene ID (NCBI) | 4067 |
| RRID | AB_2919237 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07948 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 LYN antibody CL488-60211 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
