• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 11415-1-AP

    WB analysis using 11415-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11415-1-AP (LYPD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 11415-1-AP

    IHC staining of human ovary cancer using 11415-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11415-1-AP (LYPD1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LYPD1 Polyclonal antibody

LYPD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11415-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

PSEC0181, PHTS, LYPDC1, Lynx2, Ly6/PLAUR domain-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11415-1-AP targets LYPD1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 LYPD1 fusion protein Ag1965 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LY6/PLAUR domain containing 1
别名 PSEC0181, PHTS, LYPDC1, Lynx2, Ly6/PLAUR domain-containing protein 1
计算分子量 141 aa, 15 kDa
观测分子量15 kDa
GenBank蛋白编号BC017318
基因名称 LYPD1
Gene ID (NCBI) 116372
RRIDAB_3085372
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N2G4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LY6/PLAUR domain containing 1 (LYPD1) is a highly conserved glycosylphosphatidylinositol (GPI)-anchored protein. LYPD1 contains one cysteine-rich extracellular leukocyte antigen-6 (Ly6)/uPAR (PLAUR) domain followed by signal sequence that triggers posttranslational GPI-anchor addition incorporating mature, LYPD1 into the plasma membrane. LYPD1 is believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. LYPD1 increases receptor desensitization in vitro and decreases affinity for ACh of alpha-4:beta-2-containing nAChRs. LYPD1 has 4 isoforms produced by alternative splicing (PMID: 33536191).

实验方案

Product Specific Protocols
WB protocol for LYPD1 antibody 11415-1-APDownload protocol
IHC protocol for LYPD1 antibody 11415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols