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LYPD1 Polyclonal antibody

LYPD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11415-1-AP

Print datasheet

Synonyms

PSEC0181, PHTS, LYPDC1, Lynx2, Ly6/PLAUR domain-containing protein 1

验证数据展示

  • WB analysis using 11415-1-AP

    WB analysis using 11415-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11415-1-AP (LYPD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 11415-1-AP

    IHC staining of human ovary cancer using 11415-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11415-1-AP (LYPD1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11415-1-AP targets LYPD1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen LYPD1 fusion protein Ag1965 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LY6/PLAUR domain containing 1
Synonyms PSEC0181, PHTS, LYPDC1, Lynx2, Ly6/PLAUR domain-containing protein 1
Calculated Molecular Weight 141 aa, 15 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC017318
Gene Symbol LYPD1
Gene ID (NCBI) 116372
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N2G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LY6/PLAUR domain containing 1 (LYPD1) is a highly conserved glycosylphosphatidylinositol (GPI)-anchored protein. LYPD1 contains one cysteine-rich extracellular leukocyte antigen-6 (Ly6)/uPAR (PLAUR) domain followed by signal sequence that triggers posttranslational GPI-anchor addition incorporating mature, LYPD1 into the plasma membrane. LYPD1 is believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. LYPD1 increases receptor desensitization in vitro and decreases affinity for ACh of alpha-4:beta-2-containing nAChRs. LYPD1 has 4 isoforms produced by alternative splicing (PMID: 33536191).

实验方案

Product Specific Protocols
WB protocol for LYPD1 antibody 11415-1-APDownload protocol
IHC protocol for LYPD1 antibody 11415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols