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验证数据展示

  • WB analysis using 68475-1-Ig

    WB analysis using 68475-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68475-1-Ig (Lamin B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68475-1-Ig

    IF Staining of HepG2 using 68475-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (68475-1-Ig, Clone: 2C7B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

Lamin B2 Monoclonal antibody

Lamin B2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68475-1-Ig

产品说明书

CloneNo. 2C7B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

LMNB2, LMN2, Lamin-B2, LAMB2, 2C7B1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Lamin B2 Ready-To-Use IHC Kit. Lamin B2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68475-1-Ig targets Lamin B2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Lamin B2 fusion protein Ag33296 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lamin B2
别名 LMNB2, LMN2, Lamin-B2, LAMB2, 2C7B1
计算分子量 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC006551
基因名称 Lamin B2
Gene ID (NCBI) 84823
RRIDAB_3085186
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ03252
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

实验方案

Product Specific Protocols
WB protocol for Lamin B2 antibody 68475-1-IgDownload protocol
IF protocol for Lamin B2 antibody 68475-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols