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Lamin B2 Recombinant antibody, PBS Only

Lamin B2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230184E7

Cat no : 82801-2-PBS

Print datasheet

Synonyms

LMNB2, LMN2, Lamin-B2, LAMB2, 230184E7

验证数据展示

  • WB analysis using 82801-2-RR (same clone as 82801-2-PBS)

    WB analysis using 82801-2-RR (same clone as 82801-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82801-2-RR (Lamin B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82801-2-RR (same clone as 82801-2-PBS)

    IF Staining of HepG2 using 82801-2-RR (same clone as 82801-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (82801-2-RR, Clone: 230184E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 82801-2-RR (same clone as 82801-2-PBS)

    IHC staining of human stomach cancer using 82801-2-RR (same clone as 82801-2-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82801-2-RR (Lamin B2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82801-2-RR (same clone as 82801-2-PBS)

    FC experiment of HepG2 using 82801-2-RR (same clone as 82801-2-PBS)

    (blue)1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human Lamin B2 (82801-2-RR, Clone:230184E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82801-2-RR

    Affinity and Kinetic Characterization of 82801-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82801-2-RR against Human Lamin B2 were performed. The affinity constant is 2.81 nM.

产品信息

82801-2-PBS targets Lamin B2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Lamin B2 fusion protein Ag1335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lamin B2
Synonyms LMNB2, LMN2, Lamin-B2, LAMB2, 230184E7
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC006551
Gene Symbol Lamin B2
Gene ID (NCBI) 84823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03252
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).