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Lamin B2 Recombinant antibody

Lamin B2 Recombinant Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

230184E7

Cat No : 82801-2-RR

Print datasheet

Synonyms

LAMB2, lamin B2, LMN2, LMNB2

验证数据展示

  • WB analysis using 82801-2-RR

    WB analysis using 82801-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82801-2-RR (Lamin B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82801-2-RR

    IF Staining of HepG2 using 82801-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (82801-2-RR, Clone: 230184E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, HL-60 cells, Jurkat cells, HT-1080 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82801-2-RR targets Lamin B2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Tested Reactivity Human
Immunogen Lamin B2 fusion protein Ag1335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lamin B2
Synonyms LAMB2, lamin B2, LMN2, LMNB2
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC006551
Gene Symbol LMNB2
Gene ID (NCBI) 84823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03252
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

实验方案

Product Specific Protocols
WB protocol for Lamin B2 antibody 82801-2-RRDownload protocol
IF protocol for Lamin B2 antibody 82801-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols