验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse spleen tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-10677 targets Lumican in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Lumican fusion protein Ag1106 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lumican |
| Synonyms | LUM, SLRR2D, LDC, KSPG lumican |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 65-90 kDa |
| GenBank Accession Number | BC007038 |
| Gene Symbol | Lumican |
| Gene ID (NCBI) | 4060 |
| RRID | AB_3083883 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51884 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lumican (LUM), a member of the small leucine-rich proteoglycan (SLRP) family, is one of the major extracellular components in interstitial collagenous matrices of the corneal stroma, aorta, skin, skeletal muscle, lung, kidney, bone, cartilage, and intervertebral discs. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins and have important effects on cell behavior. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and may exist as a glycoprotein in the connective tissues of other organs. Posttranslational modifications lumican goes through can increase its apparent molecular weight to 50-90 kDa. Studies show that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. (PMID: 18949819; 22252641; 9761754; 22365843)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Lumican antibody CL488-10677 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |