验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | Human Colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
10971-1-AP targets MAB21L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | MAB21L2 fusion protein Ag1423 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mab-21-like 2 (C. elegans) |
| 别名 | Protein mab-21-like 2, Protein mab 21 like 2, mab 21 like 2 (C. elegans) |
| 计算分子量 | 41 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC009983 |
| 基因名称 | MAB21L2 |
| Gene ID (NCBI) | 10586 |
| RRID | AB_2139234 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y586 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mab-21 gene has been identified as a critical component required for sensory organ identity establishment in Caenorhabditis elegans. Mab21l1 and Mab21l2 paralogs have widespread and dynamic expression patterns during vertebrate development. Depletion of Mab21l1 and Mab21l2 messages in mouse embryo arrests axial turning, and impairs notochord and neural tube differentiation. Human MAB21L2 gene encodes mab-21-like 2 protein belonging to the mab-21 family. The Mab21l2-deficient mice have defects in eye and body wall formation, indicating that Mab21l2 plays crucial roles in retina and in ventral body wall formation. MAB21L2 plays a crucial role in both heart and liver development during mouse embryogenesis. This antibody is supposed to recognize both MAB21L2 and its homolog MAB21L1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAB21L2 antibody 10971-1-AP | Download protocol |
| IHC protocol for MAB21L2 antibody 10971-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |