• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MAB21L2 Polyclonal antibody

MAB21L2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10971-1-AP

Print datasheet

Synonyms

Protein mab-21-like 2, Protein mab 21 like 2, mab 21 like 2 (C. elegans)

验证数据展示

  • WB analysis of mouse brain using 10971-1-AP

    WB analysis of mouse brain using 10971-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10971-1-AP (MAB21L2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of Human Colon cancer using 10971-1-AP

    IHC staining of Human Colon cancer using 10971-1-AP

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 10971-1-AP (MAB21L2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inHuman Colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10971-1-AP targets MAB21L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MAB21L2 fusion protein Ag1423 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mab-21-like 2 (C. elegans)
Synonyms Protein mab-21-like 2, Protein mab 21 like 2, mab 21 like 2 (C. elegans)
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC009983
Gene Symbol MAB21L2
Gene ID (NCBI) 10586
RRIDAB_2139234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mab-21 gene has been identified as a critical component required for sensory organ identity establishment in Caenorhabditis elegans. Mab21l1 and Mab21l2 paralogs have widespread and dynamic expression patterns during vertebrate development. Depletion of Mab21l1 and Mab21l2 messages in mouse embryo arrests axial turning, and impairs notochord and neural tube differentiation. Human MAB21L2 gene encodes mab-21-like 2 protein belonging to the mab-21 family. The Mab21l2-deficient mice have defects in eye and body wall formation, indicating that Mab21l2 plays crucial roles in retina and in ventral body wall formation. MAB21L2 plays a crucial role in both heart and liver development during mouse embryogenesis. This antibody is supposed to recognize both MAB21L2 and its homolog MAB21L1.

实验方案

Product Specific Protocols
WB protocol for MAB21L2 antibody 10971-1-APDownload protocol
IHC protocol for MAB21L2 antibody 10971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancers (Basel)

STAMP2 Expression Mediated by Cytokines Attenuates Their Growth-Limiting Effects in Prostate Cancer Cells.

Authors - Nicklas Pihlstrøm
View Article