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验证数据展示

  • WB analysis using 67100-1-Ig (same clone as 67100-1-PBS)

    WB analysis using 67100-1-Ig (same clone as 67100-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67100-1-Ig (MAD2L2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 67100-1-Ig (same clone as 67100-1-PBS)

    IHC staining of human lymphoma using 67100-1-Ig (same clone as 67100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67100-1-Ig (MAD2L2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAD2L2 antibody included
  • FC experiment of K-562 using 67100-1-Ig (same clone as 67100-1-PBS)

    FC experiment of K-562 using 67100-1-Ig (same clone as 67100-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human MAD2L2 (67100-1-Ig, Clone:2A4C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

MAD2L2 Monoclonal antibody, PBS Only

MAD2L2 Monoclonal Antibody for Indirect ELISA, IHC, WB
Cat No. 67100-1-PBS

产品说明书

CloneNo. 2A4C2

宿主/亚型

Mouse / IgG2b

种属反应性

rat, mouse, human

应用

Indirect ELISA, IHC, WB

别名

Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAD2L2 Ready-To-Use IHC Kit. MAD2L2 primary antibody included.

产品信息

67100-1-PBS targets MAD2L2 in Indirect ELISA, IHC, WB applications and shows reactivity with rat, mouse, human samples.

经测试应用 Indirect ELISA, IHC, WB Application Description
经测试反应性 rat, mouse, human
免疫原 MAD2L2 fusion protein Ag28233 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 MAD2 mitotic arrest deficient-like 2 (yeast)
别名 Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7
计算分子量 211 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC015244
基因名称 MAD2L2
Gene ID (NCBI) 10459
RRIDAB_2882405
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UI95
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.