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验证数据展示

  • WB analysis using 67100-1-Ig

    WB analysis using 67100-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67100-1-Ig (MAD2L2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 67100-1-Ig

    IHC staining of human lymphoma using 67100-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67100-1-Ig (MAD2L2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAD2L2 antibody included
  • FC experiment of K-562 using 67100-1-Ig

    FC experiment of K-562 using 67100-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human MAD2L2 (67100-1-Ig, Clone:2A4C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

MAD2L2 Monoclonal antibody

MAD2L2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67100-1-Ig

产品说明书

CloneNo. 2A4C2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy MAD2L2 Ready-To-Use IHC Kit. MAD2L2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67100-1-Ig targets MAD2L2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAD2L2 fusion protein Ag28233 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 MAD2 mitotic arrest deficient-like 2 (yeast)
别名 Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7
计算分子量 211 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC015244
基因名称 MAD2L2
Gene ID (NCBI) 10459
RRIDAB_2882405
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UI95
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.

实验方案

Product Specific Protocols
WB protocol for MAD2L2 antibody 67100-1-IgDownload protocol
IHC protocol for MAD2L2 antibody 67100-1-IgDownload protocol
FC protocol for MAD2L2 antibody 67100-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols