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验证数据展示

  • FC experiment of K-562 using CL488-67100

    FC experiment of K-562 using CL488-67100

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MAD2L2 Monoclonal antibody (CL488-67100, Clone:2A4C2)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated MAD2L2 Monoclonal antibody

MAD2L2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67100
CloneNo. 2A4C2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy MAD2L2 Ready-To-Use IHC Kit. MAD2L2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67100-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67100 targets MAD2L2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen MAD2L2 fusion protein Ag28233 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name MAD2 mitotic arrest deficient-like 2 (yeast)
Synonyms Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC015244
Gene Symbol MAD2L2
Gene ID (NCBI) 10459
RRIDAB_3084308
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UI95
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MAD2L2 antibody CL488-67100Download protocol
Standard Protocols
Click here to view our Standard Protocols