验证数据展示
经过测试的应用
Positive FC (Intra) detected in | K-562 cells |
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67100 targets MAD2L2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | MAD2L2 fusion protein Ag28233 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | MAD2 mitotic arrest deficient-like 2 (yeast) |
Synonyms | Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7 |
Calculated Molecular Weight | 211 aa, 24 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | BC015244 |
Gene Symbol | MAD2L2 |
Gene ID (NCBI) | 10459 |
RRID | AB_3084308 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9UI95 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 MAD2L2 antibody CL488-67100 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |