• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83267-1-RR (same clone as 83267-1-PBS)

    WB analysis using 83267-1-RR (same clone as 83267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83267-1-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83267-1-RR (same clone as 83267-1-PBS)

    FC experiment of U-251 using 83267-1-RR (same clone as 83267-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-1-RR, Clone:240097B2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00233-1

    Cytometric bead array standard curve of MP00233-1

    Cytometric bead array standard curve ofMP00233-1, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-1-PBS. Detection antibody: 83267-3-PBS. Standard: Ag27150. Range: 2.5-100 ng/mL.

  • Cytometric bead array standard curve of MP00233-2

    Cytometric bead array standard curve of MP00233-2

    Cytometric bead array standard curve ofMP00233-2, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-1-PBS. Detection antibody: 83267-2-PBS. Standard: Ag27150. Range: 1.25-100 ng/mL.

  • Affinity and Kinetic Characterization of 83267-1-RR

    Affinity and Kinetic Characterization of 83267-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83267-1-RR against Human MAEA were performed. The affinity constant is 0.496 nM.

MAEA Recombinant antibody, PBS Only (Capture)

MAEA Uni-rAbTM Recombinant Antibody for Indirect ELISA, Cytometric bead array, WB
Cat No. 83267-1-PBS

产品说明书

CloneNo. 240097B2

宿主/亚型

Rabbit / IgG

种属反应性

mouse, human

应用

Indirect ELISA, Cytometric bead array, WB

别名

EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy MAEA Ready-To-Use IHC Kit. MAEA primary antibody included.

产品信息

83267-1-PBS targets MAEA as part of a matched antibody pair:

MP00233-1: 83267-1-PBS capture and 83267-3-PBS detection (validated in Cytometric bead array)

MP00233-2: 83267-1-PBS capture and 83267-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Indirect ELISA, Cytometric bead array, WB Application Description
经测试反应性 mouse, human
免疫原 MAEA fusion protein Ag27150 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
亲和力
KD<1.00 x 10-12M
KOff<1.00 x 10-6M
KOn=9.49 x 104M
全称 macrophage erythroblast attacher
别名 EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein
计算分子量 45 kDa
GenBank蛋白编号BC001225
基因名称 MAEA
Gene ID (NCBI) 10296
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7L5Y9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C