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MAEA Recombinant antibody, PBS Only (Capture)

MAEA Uni-rAbTM Recombinant Antibody for Indirect ELISA, Cytometric bead array, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, Cytometric bead array, WB

Conjugate

Unconjugated

CloneNo.

240097B2

Cat no : 83267-1-PBS

Print datasheet

Synonyms

EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein

验证数据展示

  • WB analysis using 83267-1-RR (same clone as 83267-1-PBS)

    WB analysis using 83267-1-RR (same clone as 83267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83267-1-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83267-1-RR (same clone as 83267-1-PBS)

    FC experiment of U-251 using 83267-1-RR (same clone as 83267-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-1-RR, Clone:240097B2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00233-1

    Cytometric bead array standard curve of MP00233-1

    Cytometric bead array standard curve ofMP00233-1, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-1-PBS. Detection antibody: 83267-3-PBS. Standard: Ag27150. Range: 2.5-100 ng/mL.

  • Cytometric bead array standard curve of MP00233-2

    Cytometric bead array standard curve of MP00233-2

    Cytometric bead array standard curve ofMP00233-2, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-1-PBS. Detection antibody: 83267-2-PBS. Standard: Ag27150. Range: 1.25-100 ng/mL.

  • Affinity and Kinetic Characterization of 83267-1-RR

    Affinity and Kinetic Characterization of 83267-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83267-1-RR against Human MAEA were performed. The affinity constant is 0.496 nM.

Planning an IHC experiment? We recommend our IHCeasy MAEA Ready-To-Use IHC Kit. MAEA primary antibody included.

产品信息

83267-1-PBS targets MAEA as part of a matched antibody pair:

MP00233-1: 83267-1-PBS capture and 83267-3-PBS detection (validated in Cytometric bead array)

MP00233-2: 83267-1-PBS capture and 83267-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Indirect ELISA, Cytometric bead array, WB Application Description
Tested Reactivity mouse, human
Immunogen MAEA fusion protein Ag27150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD<1.00 x 10-12M
KOff<1.00 x 10-6M
KOn=9.49 x 104M
Full Name macrophage erythroblast attacher
Synonyms EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC001225
Gene Symbol MAEA
Gene ID (NCBI) 10296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L5Y9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C