验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells, C2C12 cells, U2OS cells, A549 cells, HeLa cells, MCF-7 cells |
| Positive FC (Intra) detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83267-1-RR targets MAEA in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | MAEA fusion protein Ag27150 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 亲和力 | KD<1.00 x 10-12M KOff<1.00 x 10-6M KOn=9.49 x 104M |
| 全称 | macrophage erythroblast attacher |
| 别名 | EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein |
| 计算分子量 | 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC001225 |
| 基因名称 | MAEA |
| Gene ID (NCBI) | 10296 |
| RRID | AB_3670940 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q7L5Y9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAEA antibody 83267-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |