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MAEA Recombinant antibody, PBS Only (Capture)

MAEA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240097E6

Cat no : 83267-4-PBS

Print datasheet

Synonyms

EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein

验证数据展示

  • WB analysis using 83267-4-RR (same clone as 83267-4-PBS)

    WB analysis using 83267-4-RR (same clone as 83267-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83267-4-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

    IF Staining of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAEA antibody (83267-4-RR, Clone: 240097E6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

    FC experiment of HeLa using 83267-4-RR (same clone as 83267-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-4-RR, Clone:240097E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00233-3

    Cytometric bead array standard curve of MP00233-3

    Cytometric bead array standard curve of MP00233-3, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-4-PBS. Detection antibody: 83267-2-PBS. Standard: Ag27150. Range: 2.5-100 ng/mL.

  • Affinity and Kinetic Characterization of 83267-4-RR

    Affinity and Kinetic Characterization of 83267-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83267-4-RR against Human MAEA were performed. The affinity constant is 0.283 nM.

Planning an IHC experiment? We recommend our IHCeasy MAEA Ready-To-Use IHC Kit. MAEA primary antibody included.

产品信息

83267-4-PBS targets MAEA as part of a matched antibody pair:

MP00233-3: 83267-4-PBS capture and 83267-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MAEA fusion protein Ag27150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name macrophage erythroblast attacher
Synonyms EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein
Calculated Molecular Weight 45 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC001225
Gene Symbol MAEA
Gene ID (NCBI) 10296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L5Y9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).